The profile of antibiotic resistance genes in pig manure composting shaped by composting stage: Mesophilic-thermophilic and cooling-maturation stages.
Identifieur interne : 000059 ( Main/Exploration ); précédent : 000058; suivant : 000060The profile of antibiotic resistance genes in pig manure composting shaped by composting stage: Mesophilic-thermophilic and cooling-maturation stages.
Auteurs : Rukun Cao [République populaire de Chine] ; Jian Wang [République populaire de Chine] ; Weiwei Ben [République populaire de Chine] ; Zhimin Qiang [République populaire de Chine]Source :
- Chemosphere [ 1879-1298 ] ; 2020.
Descripteurs français
- KwdFr :
- Agaricales (MeSH), Animaux (MeSH), Antibactériens (MeSH), Bactéries (effets des médicaments et des substances chimiques), Compostage (MeSH), Fumier (analyse), Gènes bactériens (effets des médicaments et des substances chimiques), Intégrons (MeSH), Résistance microbienne aux médicaments (génétique), Suidae (MeSH), Zea mays (effets des médicaments et des substances chimiques).
- MESH :
- analyse : Fumier.
- effets des médicaments et des substances chimiques : Bactéries, Gènes bactériens, Zea mays.
- génétique : Résistance microbienne aux médicaments.
- Agaricales, Animaux, Antibactériens, Compostage, Intégrons, Suidae.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Manure.
- chemical : Anti-Bacterial Agents.
- drug effects : Bacteria, Genes, Bacterial, Zea mays.
- genetics : Drug Resistance, Microbial.
- Agaricales, Animals, Composting, Integrons, Swine.
Abstract
The variation of antibiotic resistance genes (ARGs) and influential factors in pig manure composting were investigated by conducting simulated composting tests using four different supplement materials (wheat straw, corn straw, poplar sawdust and spent mushroom). The results show that the relative abundance of total ARGs increased by 0.19-1.61 logs after composting, and tetX, sulI, sulII, dfrA1 and aadA were the major contributors. The variations of ARG profiles and bacterial communities throughout the composting were clearly divided into mesophilic-thermophilic and cooling-maturation stages in all tests, while different supplement materials did not exert a noticeable influence. Network analysis demonstrated the diversity of bacterial hosts for ARGs, the existence of multiple antibiotic resistant bacteria, and the weak correlations between ARGs and physicochemical factors in the composting piles. Of note, integron intI1 and Mycobacterium (a potential pathogen) were positively correlated with eight and four ARGs, respectively, that displayed increased abundance after composting.
DOI: 10.1016/j.chemosphere.2020.126181
PubMed: 32109697
Affiliations:
Links toward previous steps (curation, corpus...)
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Electronic address: wwben@rcees.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 18 Shuang-qing Road, Beijing, 100085</wicri:regionArea><wicri:noRegion>100085</wicri:noRegion></affiliation></author><author><name sortKey="Qiang, Zhimin" sort="Qiang, Zhimin" uniqKey="Qiang Z" first="Zhimin" last="Qiang">Zhimin Qiang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 18 Shuang-qing Road, Beijing, 100085, China. Electronic address: qiangz@rcees.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 18 Shuang-qing Road, Beijing, 100085</wicri:regionArea><wicri:noRegion>100085</wicri:noRegion></affiliation></author></analytic><series><title level="j">Chemosphere</title><idno type="eISSN">1879-1298</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agaricales (MeSH)</term><term>Animals (MeSH)</term><term>Anti-Bacterial Agents (MeSH)</term><term>Bacteria (drug effects)</term><term>Composting (MeSH)</term><term>Drug Resistance, Microbial (genetics)</term><term>Genes, Bacterial (drug effects)</term><term>Integrons (MeSH)</term><term>Manure (analysis)</term><term>Swine (MeSH)</term><term>Zea mays (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agaricales (MeSH)</term><term>Animaux (MeSH)</term><term>Antibactériens (MeSH)</term><term>Bactéries (effets des médicaments et des substances chimiques)</term><term>Compostage (MeSH)</term><term>Fumier (analyse)</term><term>Gènes bactériens (effets des médicaments et des substances chimiques)</term><term>Intégrons (MeSH)</term><term>Résistance microbienne aux médicaments (génétique)</term><term>Suidae (MeSH)</term><term>Zea mays (effets des médicaments et des substances chimiques)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Manure</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Anti-Bacterial Agents</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Fumier</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Bacteria</term><term>Genes, Bacterial</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Bactéries</term><term>Gènes bactériens</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Drug Resistance, Microbial</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Résistance microbienne aux médicaments</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Agaricales</term><term>Animals</term><term>Composting</term><term>Integrons</term><term>Swine</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Agaricales</term><term>Animaux</term><term>Antibactériens</term><term>Compostage</term><term>Intégrons</term><term>Suidae</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The variation of antibiotic resistance genes (ARGs) and influential factors in pig manure composting were investigated by conducting simulated composting tests using four different supplement materials (wheat straw, corn straw, poplar sawdust and spent mushroom). The results show that the relative abundance of total ARGs increased by 0.19-1.61 logs after composting, and tetX, sulI, sulII, dfrA1 and aadA were the major contributors. The variations of ARG profiles and bacterial communities throughout the composting were clearly divided into mesophilic-thermophilic and cooling-maturation stages in all tests, while different supplement materials did not exert a noticeable influence. Network analysis demonstrated the diversity of bacterial hosts for ARGs, the existence of multiple antibiotic resistant bacteria, and the weak correlations between ARGs and physicochemical factors in the composting piles. Of note, integron intI1 and Mycobacterium (a potential pathogen) were positively correlated with eight and four ARGs, respectively, that displayed increased abundance after composting.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">32109697</PMID><DateCompleted><Year>2020</Year><Month>05</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>05</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1298</ISSN><JournalIssue CitedMedium="Internet"><Volume>250</Volume><PubDate><Year>2020</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Chemosphere</Title><ISOAbbreviation>Chemosphere</ISOAbbreviation></Journal><ArticleTitle>The profile of antibiotic resistance genes in pig manure composting shaped by composting stage: Mesophilic-thermophilic and cooling-maturation stages.</ArticleTitle><Pagination><MedlinePgn>126181</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0045-6535(20)30374-X</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.chemosphere.2020.126181</ELocationID><Abstract><AbstractText>The variation of antibiotic resistance genes (ARGs) and influential factors in pig manure composting were investigated by conducting simulated composting tests using four different supplement materials (wheat straw, corn straw, poplar sawdust and spent mushroom). The results show that the relative abundance of total ARGs increased by 0.19-1.61 logs after composting, and tetX, sulI, sulII, dfrA1 and aadA were the major contributors. The variations of ARG profiles and bacterial communities throughout the composting were clearly divided into mesophilic-thermophilic and cooling-maturation stages in all tests, while different supplement materials did not exert a noticeable influence. Network analysis demonstrated the diversity of bacterial hosts for ARGs, the existence of multiple antibiotic resistant bacteria, and the weak correlations between ARGs and physicochemical factors in the composting piles. Of note, integron intI1 and Mycobacterium (a potential pathogen) were positively correlated with eight and four ARGs, respectively, that displayed increased abundance after composting.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Rukun</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 18 Shuang-qing Road, Beijing, 100085, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jian</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Institute of Rural Energy and Environmental Protection, Academy of Agricultural Planning and Engineering, Ministry of Agriculture and Rural Affairs, Beijing, 100121, China; Key Laboratory of Technologies and Models for Cyclic Utilization from Agricultural Resources, Ministry of Agriculture and Rural Affairs, Beijing, 100121, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ben</LastName><ForeName>Weiwei</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 18 Shuang-qing Road, Beijing, 100085, China. Electronic address: wwben@rcees.ac.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qiang</LastName><ForeName>Zhimin</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 18 Shuang-qing Road, Beijing, 100085, China. 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